HELenic LAboratories
& Services of CHEMistry & Quality Management

Indicative list of testing for feed raw materials:

Silages

Method

Method id

Method reference

Equipment

Select offer

Moisture - dry matter Μ01/F8 Reg. 152/2009/EC Annex III, meth.A  

Ash Μ02/F8 Reg. 152/2009/EC Annex III, meth.M  

Volatile components Μ35/F8 In house method  

Protein crude Μ03/F8 In house method based on Reg. 152/2009/ΕC Annex  ΙΙΙ, meth. C & on ISO 1871  

Ammonium nitrogen Μ32/F8 In house method Titration

pH Μ33/F8 In house method pH-meter

Water soluble carbohydrates Μ59/F8 In house method  

Starch Μ15a/F8 Luff - Scoorl Titration

Ether extract Μ24/F8 In house method Soxhlet

Acid DetergentFibre (ADF) Μ18/F8 ISO 13906  

Lactic acid Μ62/F8 In house method HPLC/UV

Acetic acid Μ63/F8 In house method HPLC/UV

Butyric acid Μ64/F8 In house method HPLC/UV

Metabolisable Energy Μ34/F8 In house method  

Digestibility Μ36/F8 In house method  

Nitrate Μ71/F8 In house method Spectrophotometer

Sugar total Μ04/F8 Reg. 152/2009/EC Annex III, meth.J  

 

Soy

Testing

Method id

Method

Equipment

Select offer

Moisture - dry matter Μ01/F8 Reg. 152/2009/EC Annex III, meth.A  

Ash Μ02/F8 Reg. 152/2009/EC Annex III, meth.M  

Protein crude Μ03/F8 In house method based on Reg. 152/2009/ΕC Annex  ΙΙΙ, meth. C & on ISO 1871  

Urease activity Μ17/F8 FAO method 5.1 Potentiometer

KOH protein solubility Μ26/F8 In house method Kjeldhahl

Protein Dispersibility Index (PDI) Μ29/F8 In house method Kjeldhahl

Fibre crude Μ05/F8 Gerhardt Method  

Acid DetergentFibre (ADF) Μ18/F8 ISO 13906  

Neutral Detergent Fibre (NDF) Μ19/F8 ISO 13906  

Acid Detergent Lignin (ADL) Μ20/F8 ISO 13906  

Starch Μ15a/F8 Luff - Scoorl Titration

Starch Μ15b/F8 Reg. 152/2009/EC Annex III, meth.I Polarimeter

Fats & oils crude Μ06/F8 Reg. 152/2009/EC Annex III, meth.H  

Ether extract Μ24/F8 In house method Soxhlet

Fatty acids profile (FAMEs) Μ07/F8 ISO 17764  

Thiobarbituric acid (TBA) Μ59/F8 In house method Spectrophotometer

Trypsin inhibitor activity of soya products Μ25/F8 ISO 14902  

Nitrogen soluble (in pepsin acid solution) M52/F8 ISO 6655  

Urea Μ16/F8 ISO  6654  

Amino acids free (except tryptophane) M53/F8 Reg. 152/2009/EC Annex III, meth.F  

Amino acids total (except tryptophane) M54/F8 Reg. 152/2009/EC Annex III, meth.F  

Pesticides residues Μ42/F8 ISO 14181 & ISO 14182  

Aflatoxin B1 (& B2, G1, G2) Μ43/F8 ISO 14718  

Ochratoxin A Μ44/F8 In house method HPLC/FLD

Deoxynivalenol (DON) Μ45/F8 In house method ELISA

Zearalenone (ZEN) Μ46/F8 ISO 17372  

Dioxin like PCBs (PCB 28, 52, 101, 138, 153, 180) Μ48/F8 Reg. 152/2009/EC Annex V, meth.B  

Lead (Pb) Μ49/F8 In house method AAS

Cadmium (Cd) Μ49/F8 In house method AAS

Nickel (Ni) Μ49/F8 In house method AAS

Chrome (Cr) Μ49/F8 In house method AAS

Arsenic (As) Μ49/F8 In house method AAS

Mercury (Hg) Μ50/F8 In house method AAS

Fumonisins (FB1/FB2) M130/GF In house method  

Salmonella spp detection Μ01/F1Y2 ISO 6579-1  

Yeasts and moulds (aw>0,95) Μ06/F1Y2 ISO 21528-1  

Yeasts and moulds (aw≤0,95) Μ07/F1Y2 ISO 21528-2  

 

Corn

Testing

Method id

Method

Equipment

Select offer

Moisture - dry matter Μ01/F8 Reg. 152/2009/EC Annex III, meth.A  

Ash Μ02/F8 Reg. 152/2009/EC Annex III, meth.M  

Protein crude Μ03/F8 In house method based on Reg. 152/2009/ΕC Annex  ΙΙΙ, meth. C & on ISO 1871  

Fats & oils crude Μ06/F8 Reg. 152/2009/EC Annex III, meth.H  

Fibre crude Μ05/F8 Gerhardt Method  

Acid DetergentFibre (ADF) Μ18/F8 ISO 13906  

Neutral Detergent Fibre (NDF) Μ19/F8 ISO 13906  

Starch Μ15a/F8 Luff - Scoorl Titration

Starch Μ15b/F8 Reg. 152/2009/EC Annex III, meth.I Polarimeter

Calcium Μ10/F8 ISO 6869  

Phosphorus total Μ11/F8 ISO 6491  

Lysine available Μ81/F8 ISO 5510  

Methionine Μ82/F8 In house method Spectrophotometer

Tryptophan Μ83/F8 Reg. 152/2009/EC Annex III, meth.G  

Pesticides residues Μ42/F8 ISO 14181 & ISO 14182  

Aflatoxin B1 (& B2, G1, G2) Μ43/F8 ISO 14718  

Ochratoxin A Μ44/F8 In house method HPLC/FLD

Deoxynivalenol (DON) Μ45/F8 In house method ELISA

Zearalenone (ΖΕΝ) Μ46/F8 ISO 17372  

Dioxin like PCBs (PCB  28, 52, 101, 138, 153, 180) Μ48/F8 Reg. 152/2009/EC Annex V, meth.B  

Lead (Pb) Μ49/F8 In house method AAS

Cadmium(Cd) Μ49/F8 In house method AAS

Nickel(Ni) Μ49/F8 In house method AAS

Chrome (Cr) Μ49/F8 In house method AAS

Arsenic (As) Μ49/F8 In house method AAS

Mercury (Hg) Μ50/F8 In house method AAS

Lactic acid Μ62/F8 In house method HPLC/UV

Acetic acid Μ63/F8 In house method HPLC/UV

Butyric acid Μ64/F8 In house method HPLC/UV

Mass per hectolitre M71/F1Y6 ISO 7971-1  

Mass of 1000 grains M72/F1Y6 ISO 520  

Artificial colorants detection M87/F8 In house method  

Salmonella spp detection Μ01/F1Y2 ISO 6579-1  

Lactic acid bacteria Μ13/F1Y2 ISO 15214   

 

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