HELenic LAboratories
& Services of CHEMistry & Quality Management

Indicative list of testing for Coffee – Tea – Cocoa:

Coffee – Tea – Cocoa

Method

Method id

Method reference

Equipment

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Nutritional declaration (mandatory testing according to European legislation): energy, fat, saturates, carbohydrate, sugars, protein, salt (& moisture, ash) P01/GF see General method (GF)  

Coffee

Method

Method id

Method reference

Equipment

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Caffeine M01/F1Y5 In house method HPLC-UV

Glucose total M02/F1Y5 In house method HPLC-RI

Xylose total M03/F1Y5 In house method HPLC-RI

Tanning materials M04/F1Y5 Tillmans method  

 
Starchy substances detection M05/F1Y5 Tillmans method  

 Fig coffee detection M06/F1Y5 Tillmans method  

Chicory in coffee M07/F1Y5 Tillmans method  

Caramel detection M08/F1Y5 Tillmans method  

Woody substances detection M09/F1Y5 GFDC method  

Microscopic examination M10/F1Y5    

Moisture / water content M11/F1Y5 ISO 1446  

Ash M12/F1Y5 In house method  

Water activity M13/F1Y5 In house method water activity meter

Ochratoxin Α M14/F1Y5 In house method HPLC-UV

Microscopic examination M15/F1Y5 In house method  

Furan M16/F1Y5 In house method  

Particle size M17/F1Y5 In house method sieve analysis

chlorogenic acids M18/F1Y5 DIN 10767  

16-O-methyl cafestol M19/F1Y5 DIN 10779  

pH M20/F1Y5 DIN 10776  

Sugars profile M21/F1Y5 In house method HPLC-RI

Phosphoric acid M22/F1Y5 In house method spectrophotometer

Steviol glycosides M67/GF In house method  

Microorganisms enumeration M23/F1Y5 ISO 4833  

Coliforms enumeration M24/F1Y5 ISO 4831  

Yeasts and moulds (aw≤0,95) Μ07/F1Y2 ISO 21528-2  

Salmonella spp detection Μ01/F1Y2 ISO 6579-1  

Tea

Method

Method id

Method reference

Equipment

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Water extract M31/F1Y5 ISO 9768  

Moisture / loss in mass at 105C M32/F1Y5 ISO 1573  

Ash total M33/F1Y5 ISO 1575  

Ash water-soluble and ash water-insoluble M34/F1Y5 ISO 1576  

Ash acid-insoluble M35/F1Y5 ISO 1577  

Alkalinity of water-soluble ash M36/F1Y5 ISO 1578  

Caffeine M37/F1Y5 ISO 10727  

Particle size analysis M38/F1Y5 ISO 11286  

Microscope analysis M39/F1Y5    

Crude fibre M40/F1Y5 ISO 15598  

Polyphenols (total) M41/F1Y5 ISO 14502-1  

Catechins  M42/F1Y5 In house method HPLC-UV

Antioxidant capacity M43/F1Y5 In house method  

Iron Fe M44/F1Y5 In house method AAS

Copper Cu M45/F1Y5 In house method AAS

Lead Pb M46/F1Y5 In house method AAS

Zinc Zn M47/F1Y5 In house method AAS

Cadmium Cd M48/F1Y5 In house method AAS

pesticides M49/F1Y5 In house method GC ECD/NPD/FPD

anthraquinone M50/F1Y5 In house method  

pyrrolizidine alkaloids M51/F1Y5 In house method  

Coumarin M52/F1Y5 In house method  

Sorbic acid M45/GF In house method HPLC-UV

Benzoic acid M46/GF In house method HPLC-UV

Phosphorus Ρ Μ11/GF In house method Spectrophotometer

Steviol glycosides M67/GF In house method  

Bacillus cereus (low numbers)) M24/F1Y2 ISO 21871  

Cocoa

Method

Method id

Method reference

Equipment

Select offer

Moisture M61/F1Y5 In house method  

Στερεά Cocoa M62/F1Y5    

Ξηρά στερεά Cocoa M63/F1Y5    

Butter of cocoa M64/F1Y5 In house method  

Melting point of fats M65/F1Y5 In house method  

Melting point of fatty acids Μ05/F1Y3 In house method  

Fatty Acid Methyl Esters (FAMEs) M20/F1Y3 Reg. 2568/1991/EEC  

Iodine value M18/F1Y3 Reg. 2568/1991/EEC  

Saponification value M18/F1Y3 ISO 3657  

Ash total M66/F1Y5 In house method Furnace

Ash acid-insoluble M67/F1Y5 In house method Furnace

Benzo(a)pyrene M68/F1Y5 In house method HPLC-FLD

PAH (Polycyclic aromatic hydrocarbons) M69/F1Y5 In house method HPLC-FLD

Gluten M70/F1Y5 In house method ELISA

Cadmium Cd M71/F1Y5 In house method AAS

Colour additive detection M72/F1Y5 In house method TLC

Theobromine M73/F1Y5 In house method HPLC-UV

Cellulose Μ01/F1Y2 In house method Gravimetric method

Salmonella spp detection Μ01/F1Y2 ISO 6579-1  

Sorbic acid M45/GF In house method  

Phosphorus Ρ Μ11/GF In house method Spectrophotometer

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